Mutations can be entered using either the text box or pull down menus.
To use the text box, type each mutation in uppercase separated by one or more spaces (the consensus wildtype amino acids and separating commas are optional). If there is a mixture of more than one amino acid at a position, write both amino acids (an intervening slash is optional). Use lowercase "ins" to indicate an insertion and lowercase "del" to indicate a deletion; lowercase mutations other than "ins" or "del" will cause the program to print a Javascript warning.
The pulldown menus are implemented with Javascript. Each pulldown menu lists amino acids known to be associated with drug resistance as well as amino acids at drug-resistance positions that occur with a frequency of >=0.1% among treated or untreated patients. To enter an amino acid not in the list, click on the asterisk "*". If you have trouble using Javascript on your browser or if you wish to enter a mixture, you should use the textbox instead.
The output can be customized to display mutation comments, mutation scores, and an optional identifier and date. For further explanations and sample datasets please see the Release Notes.
